Plasma-SeqSensei™ Non-Small Cell Lung Cancer RUO Kit

The Plasma-SeqSensei™ NSCLC RUO (research use only) Kit detects mutations in circulating tumour DNA (ctDNA) from plasma in patients with non-small cell lung cancer.

The kit uses next generation sequencing for the detection and identification of mutations in BRAF, EGFR, KRAS, and PIK3CA genes in human cfDNA isolated from blood plasma which play a significant role in the development of NSCLC and are relevant biomarkers for prognosis, choice of therapy as well as monitoring of recurrence and therapy response. [1] [2]   

EGFR gene mutations (in exons 18-21) coding for the internal receptor tyrosine kinase [TK] domain of EGFR have a variable ability to activate the TK in the absence of ligand binding. EGFR mutations are reported in 10-15% of Caucasian adenocarcinomas (all cases regardless of smoking history) and in 40–60% of adenocarcinomas in East Asian populations. [3]

BRAF mutations are the driver oncogenes in 1-3% of cases of NSCLC (classic V600E form (50%)). [4]

KRAS mutations occur in ~30% of lung adenocarcinomas (KRAS G12C comprises ~44% of all KRAS mutations, so finally making up mutations in ~13% of all lung adenocarcinomas cases). [5]

PIK3CA mutations have been found at a frequency of 2-7% in NSCLC (in exon 9 and exon 20). [6]

For the configuration of the Plasma-SeqSensei™ NSCLC RUO kits, four key cancer genes were selected after comparison of mutation frequencies using the COSMIC (Catalogue of Somatic Mutation in Cancer) and cBioPortal for cancer genomics databases.